Zeiss Cell Discoverer 7 LSM 900 (Ramaciotti)

The Zeiss Celldiscoverer 7 is a boxed imaging system suited for performing automated experiments. This inverted widefield system features automatic focusing, focus stabilisation, time-course and position setup to allow researchers to insert a sample and set up long-term experiments in minutes

The Celldiscoverer 7 has a fully temperature-controlled sample chamber with a CO2 pump, this chamber is highly stable and allows for live cells to stay healthier for longer. By utilising LEDs as a light-source for widefield imaging, live-cells can be imaged gently and with low phototoxicity, making the Celldiscoverer 7 ideal for long-term live experiments. Additionally, an infrared LED is used for transmitted light brightfield and phase contrast imaging through all available objectives and through a variety of sample holders.

This system is fitted with 4 objectives which allow imaging through both plastic and glass sample holders. The higher magnification objectives are suited to acquiring high-resolution images through glass. A separate magnification changer expands the obtainable magnification range to 2.5x-100x. The water-immersion 50x objective allows for detailed imaging and is supported in automated experiments by an automatic immersion dispenser.

In addition to its widefield capabilities, this microscope is capable of confocal imaging using an attached Zeiss LSM 900 system. This includes an Airyscan 2 module, allowing for imaging with up to 1.5x increased resolution and an improved signal-to-noise ratio. The module operates using 405, 488, 561 and 640 nm lasers and sensitive GaAsP detectors. The multiple imaging modalities of this system allows researchers to pre-screen their samples at low magnification using widefield microscopy before selecting specific regions to image at high resolution using confocal or Airyscan imaging.